Chinese Hamster Ovary (CHO) cells are widely used in the development and production of recombinant proteins. Cergentis’ Targeted Locus Amplification (TLA) technology enables targeted complete next generation sequencing (NGS) of expression constructs and integration sites and is a cost-effective and high-quality alternative to conventional methods for (CHO) cell line development and quality control. Offered both as a service and kits, TLA is used in multiple stages in CHO cell line development, e.g. early stage clone selection, and enables the assessment of genetic stability of Master Cell Banks, Working Cell Banks and End-of-Production cells.

Genedata Selector^® is a software platform of Genedata that allows researchers to manage and analyse genomic data efficiently and easily. Genedata Selector now includes TLA data analysis pipelines.

Cergentis’ Founding Director Max van Min comments:

"We are proud and happy Genedata Selector now includes tools for TLA NGS data analysis. This will help our kit customers to best utilise our TLA technology in their CHO cell line development and quality control.”

To learn more about how Genedata Selector can be used to analyse TLA data in CHO cell line development and quality control, please read the new application note: Genedata Selector for Efficient and High Quality CHO Cell Line Development: Leveraging TLA for complete sequencing of expression constructs and integration sites.

For more background information about the TLA technology in CHO cell line development please read Cergentis’ application note: Targeted complete next generation sequencing and quality control of transgenes and integration sites in CHO cell line development.